Strains Di!erentiation of Microsporum canis by RAPD Analysis Using (GACA)
نویسندگان
چکیده
Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal di"erentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal di"erentiation within M. canis as distinct from other species. !e aim of this study was application of (GACA) 4 repetitive primer and (ACA) 5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T. rubrum (CBS 120358), T. mentagrophytes (CBS 120357) and E. !occosum (CBS 970.95). !e genomic DNAs of the strains were used as a template in RAPD reaction. No di"erentiation was observed for the analyzed M. canis strains using (GACA) 4 and (ACA) 5 typing. K e y w o r d s: M. canis, dermatophytes, strains di"erentiation, RAPD method * Corresponding author: A. Dobrowolska, University of Łódź, Department of Genetics of Microorganisms; 90-237 Łódź, Poland, ul. Banacha 12/16; phone: +48 42 635-47-72; e-mail: [email protected] Dobrowolska A. et al. 2 146
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